How ETHA Uses HPLC Lab Technology

etha lab testing hplc alkaloids

Conducting Research and Development (R&D)  and lab testing through our own ETHA Labs is one way we go beyond being a simple vendor/reseller. Most kratom vendors do NOT even test their kratom before selling to the public. If they do test, it is usually a single test per large scale harvest by an outside lab. While ETHA does verify our findings through 3rd-party, independent laboratories, we manage testing ourselves. We conduct several lab test on each small batch harvest. This gives us more detailed and thorough data. This is how we are able to make consistently different kratom products for you. We can precisely measure every harvest and their alkaloids to make our products. That is why our SunRise, MidDay Gold, SunSet… are always consistently the same high quality kratom you love.   

etha lab testing kratom alkaloids

Our tireless pursuit for scientific legitimacy and superior product quality is one of the pivotal characteristics that sets ETHA apart. We continue to go beyond basic standards and minimum requirements to be the very best kratom manufacturer. Our R&D team never stops exploring new research, concepts, products and projects. One important tool our R&D team heavily makes use of is High-performance liquid chromatography (HPLC).  

We harvest our kratom from several different forests, lab measure alkaloids and use the data to determine which harvest goes into which blend. The HPLC is a vital tool to help us measure the unique alkaloids present in each harvest that comes through our facility. By seeing an output of what alkaloids and at which amounts are present in a sample, we are able to formulate and manufacture our unique premium kratom.

etha kratom harvesting

What is HPLC?

The HPLC is an analytical technique to separate, identify, and quantify components in a mixture or sample. It is perhaps, the single biggest chromatography technique essential to most laboratories worldwide. In other words, this is a way for our R&D team to separate a sample to discover the unique components or elements that compose thparticular sample. 

HPLC lab testing etha kratom

Although both the machine and the technique have many moving parts, the basic idea is as follows: A high pressure pump moves liquid and pushes it through our sample to extract its components with the help of that pressurized liquid. The pressurized liquid is typically a mixture of solvents such as water, acetonitrile and/or methanol. In column chromatography solvent drips through a column filled with an adsorbent under gravity.

HPLC is a highly improved form of column chromatography. A pump forces a solvent through a column under high pressures. The column packing material or adsorbent is typically a granular material made of solid particles such as silica or polymers.

The pressure makes the technique much faster and allows using much smaller particles for the column packing material. The smaller particles have a much greater surface area for interactions between the stationary phase and the molecules flowing past it. This results in a much better separation of the components of the mixture.

The components of a mixture are separated from each other due to their different degrees of interaction with the particles. This causes different separation times for the different components and leads to the separation of the components as they flow out the column. The result is a graph-like output of peaks of varying heights at varying time intervals.

etha lab kratom hplc graph

From HPLC to Analysis

The area under each peak is a measure of the concentration of the particular compound (component) it represents. The higher the peak, the more of that compound in the sample. So, what exactly do the HPLC results represent? What do these different peaks mean? 

We know that kratom has 40+ naturally-occurring bio-active alkaloids. Each harvest has natural variations in alkaloids due to various factors including growing season, harvest locations, and harvest criteria. Therefore, when we refer to a tested “sample” above, we are talking about a kratom extract sample. And as such, the “components” constitute the various alkaloids that make up a particular extract sample. 

The HPLC is a vital tool to help us measure the unique alkaloids present in each ETHA harvest that comes through our facility. By seeing an output of what alkaloids and at which amounts are present in a sample, we are able to formulate and manufacture our unique ETHA premium kratom.

etha natural botanicals kratom shop

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Comments (6)

  • robert smith Reply

    Well written and informative. If only all of the folks on the relevant Kratom FB groups knew about you. I promote your products, but I’m only one person 🙁

    January 13, 2021 at 3:35 am
    • The ETHA Team Reply

      Thanks so much Robert for promoting us. If you could do us a favor and share what the prominent kratom groups are on FB, we will do our best to join and share our knowledge. In the meantime, thank you for sharing it for us.

      January 13, 2021 at 10:47 am
  • Eric Reply

    Thank you for replying and clarifying .
    And I commend Etha for doing the right thing.
    I just pray to God and trust that all practices remain consistent and extremely thorough,
    Unfortunately With a product like this there is no room for error,
    As it can present serious health issues in the moment and down the road .
    And for those companies out there that do not thoroughly test this plant from Indonesia and abroad are playing with peoples health and it will eventually come back to ruin the whole industry, as well and not give it the legitimacy it deserves.

    I have also realized that many companies have the barcodes that you can scan, which makes sense and looks nice
    but what I’ve heard from someone in the
    Business side of industry is that they can be easily replicated by vendors ,
    and that it doesn’t automatically ensure transparency as it relates to results on that specific patch. ( disturbing to say the least).
    I appreciate that etha concentrates on the therapeutic aspects of this plant,
    as I see other companies focus their marketing on the euphoric aspect, which is counterproductive on the whole
    for kratom to be accepted as a viable and natural alternative to relieve pain, inflammation and provide energy..
    I believe there should be some pushback on these other companies from the legitimate ones and some oversight to protect the efficacy of the product…

    Thank you for your continued efforts to keep this interesting yet very controversial product safe and productive.


    January 12, 2021 at 4:53 pm
    • The ETHA Team Reply

      Counterfeit lab test and labels are common. So is the phrase “lab tested” without any actual testing happening. It’s become a common phrase that’s added to kratom products.

      You’ll be interested in knowing that ETHA is working on a system to securely track kratom from the forest to our customers using cutting edge hyper ledger technology and cloud interfaces. Our goal is to securely track the movement of our kratom and associate test results so our customers can know what exactly they are getting when they purchase ETHA products. People will be able to see what forest their kratom came from, what steps went into the production of the kratom, the exact test results, and where it traveled through it’s journey to become ETHA products.

      While ETHA is already leading the way with lab testing, QR code and online accessible lab test results, and unique safety measures like our proprietary kratom pasteurization process, we continue to innovate to make things even better. Stick with ETHA and you’ll be contributing to proper growth of kratom as a legitimate industry.

      January 12, 2021 at 6:04 pm
  • Eric Reply

    I appreciate the in-depth explanation on testing and separation of alkaloids to produce more nuanced products.

    But one question still remains,?

    as mentioned in this description it’s still understood that only a small batch of each large quantity is sampled.
    . I understand logistically that it may be unrealistic to sample the entire batch,
    but who’s to say that a small batch that’s sampled and even third-party verified ensures that the remaining amount of inventory is safe from contaminants????
    Assuming that the remaining batch is based on the results of a minimal amount is not that comforting…

    Please explain
    thank you,!

    January 12, 2021 at 11:21 am
    • The ETHA Team Reply

      Yes, samples harvests for test is definitely an issue. Most kratom vendors simply grab a couple grams from the top of a large ton and send to a lab. They way ETHA does things differently is how we pull samples, the amount we use for testing per harvest, and the frequency of testing. We do not just grab a small amount from a bundle. We have tools that help us to take a little bit on all corners and throughout the center of a harvest. Because we have our own HPLC lab analytical equipment and run our own ETHA Labs, we can run multiple test on the same harvest. We also group smaller quantities together in what we call a harvest or batch. Other vendors will take a ton (2,000 lbs) or more of kratom and do one test. We divide our harvests into hundreds of lbs versus thousands and do several test on the same batch. We validate our internal lab testing results with 3rd-party, independent labs and publish those results online for our customers to see.

      So, yes, just because something is lab tested does not mean it is accurate or applicable. It is how in fact the testing is done and the equipment used. This is where ETHA is better than the competition. We go beyond industry standards to ensure our customers are getting the safest and most effective kratom products.

      Here’s more info – https://ethanaturals.com/kratom-laboratory-testing-explained/

      January 12, 2021 at 1:45 pm

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