How ETHA Uses HPLC Lab Technology
Conducting Research and Development (R&D) and lab testing through our own ETHA Labs is one way we go beyond being a simple vendor/reseller. Most kratom vendors do NOT even test their kratom before selling to the public. If they do test, it is usually a single test per large scale harvest by an outside lab. While ETHA does verify our findings through 3rd-party, independent laboratories, we manage testing ourselves. We conduct several lab test on each small batch harvest. This gives us more detailed and thorough data. This is how we are able to make consistently different kratom products for you. We can precisely measure every harvest and their alkaloids to make our products. That is why our SunRise, MidDay Gold, SunSet… are always consistently the same high quality kratom you love.
Our tireless pursuit for scientific legitimacy and superior product quality is one of the pivotal characteristics that sets ETHA apart. We continue to go beyond basic standards and minimum requirements to be the very best kratom manufacturer. Our R&D team never stops exploring new research, concepts, products and projects. One important tool our R&D team heavily makes use of is High-performance liquid chromatography (HPLC).
We harvest our kratom from several different forests, lab measure alkaloids and use the data to determine which harvest goes into which blend. The HPLC is a vital tool to help us measure the unique alkaloids present in each harvest that comes through our facility. By seeing an output of what alkaloids and at which amounts are present in a sample, we are able to formulate and manufacture our unique premium kratom.
What is HPLC?
The HPLC is an analytical technique to separate, identify, and quantify components in a mixture or sample. It is perhaps, the single biggest chromatography technique essential to most laboratories worldwide. In other words, this is a way for our R&D team to separate a sample to discover the unique components or elements that compose the particular sample.
Although both the machine and the technique have many moving parts, the basic idea is as follows: A high pressure pump moves liquid and pushes it through our sample to extract its components with the help of that pressurized liquid. The pressurized liquid is typically a mixture of solvents such as water, acetonitrile and/or methanol. In column chromatography solvent drips through a column filled with an adsorbent under gravity.
HPLC is a highly improved form of column chromatography. A pump forces a solvent through a column under high pressures. The column packing material or adsorbent is typically a granular material made of solid particles such as silica or polymers.
The pressure makes the technique much faster and allows using much smaller particles for the column packing material. The smaller particles have a much greater surface area for interactions between the stationary phase and the molecules flowing past it. This results in a much better separation of the components of the mixture.
The components of a mixture are separated from each other due to their different degrees of interaction with the particles. This causes different separation times for the different components and leads to the separation of the components as they flow out the column. The result is a graph-like output of peaks of varying heights at varying time intervals.
From HPLC to Analysis
The area under each peak is a measure of the concentration of the particular compound (component) it represents. The higher the peak, the more of that compound in the sample. So, what exactly do the HPLC results represent? What do these different peaks mean?
We know that kratom has 40+ naturally-occurring bio-active alkaloids. Each harvest has natural variations in alkaloids due to various factors including growing season, harvest locations, and harvest criteria. Therefore, when we refer to a tested “sample” above, we are talking about a kratom extract sample. And as such, the “components” constitute the various alkaloids that make up a particular extract sample.
The HPLC is a vital tool to help us measure the unique alkaloids present in each ETHA harvest that comes through our facility. By seeing an output of what alkaloids and at which amounts are present in a sample, we are able to formulate and manufacture our unique ETHA premium kratom.